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The ABI 3100 Automated Sequencer The 3100 (acquired in November of 2001) is the now the preferred instrument for sequencing DNA in the Taylor Lab. The 3100 employs 16 capillaries, thus enabling one to sequence an entire 96 well plate in just under 15 hours. The 3100 is not inexpensive to operate, however, and enjoys its share of downtime, as documented in our 3100 hall of shame log. |
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The EagleEye by Stratagene The EagleEye is a fully integrated still video imaging system for gel documentation. Housed within the EagleEye is a UV light source (a transilluminator). As DNA bound with Ethidium Bromide will fluoresce when illuminated with UV light, a typical agarose gel is stained with EtBr for 10 minutes, destained in water for 15, and then placed on the transilluminator for viewing of DNA bands. The EagleEye's video camera is connected to a Macintosh II CI and an image printer. Images can be scanned directly into the computer or printed out via the high-resolution thermal printer. Click on the EagleEye to view a typical minigel. |
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