Ph.D. Biochemistry University of Colorado at Boulder, 1997
B.S. Chemical Engineering Cornell University
In my laboratory, we generate, analyze, and integrate biological information across disciplines to 1) discover host processes of importance to an interaction through the use of systems-level data, 2) uncover the process components and their regulation through detailed biochemical, molecular genetic, cell biological, and genomic analyses, and 3) elucidate the functional role of a process in the context of a given plant pathosystem using theoretical, informatic, and experimental approaches. We then assess our findings in a broader context to determine common and divergent plant host mechanisms and associated control points across pathosystems and to inform our understanding of fundamental biological processes.
We are particularly interested in integrating data from our lab with that of others to gain a holistic understanding of processes of importance to the sustained growth and reproduction of an adapted powdery mildew pathogen on Arabidopsis. Current projects in the laboratory continue our work on the regulation and function of the stress hormone salicylic acid and induced endoreduplication associated with the sustained external growth and reproduction of the powdery mildew on Arabidopsis. We also continue to employ systems-level analyses to define new host processes of importance to this pathosystem.
We thank UC Berkeley, the National Science Foundation, the Environmental Protection Agency, and the Winkler Family foundation for funding.
Okrent, R.A. and M.C. Wildermuth (2011) Evolutionary history of the GH3 family of acyl adenylases in rosids. Plant Molecular Biology, In press.
Jones, A.M. and M.C. Wildermuth (2011) The phytopathogen Pseudomonas syringae pv tomato DC3000 has three high affinity iron scavenging systems functional under iron limitation but dispensable for pathogenesis. Journal of Bacteriology 193: 2767-2775. PDF
Chandran, D., Inada, N., and M.C. Wildermuth (2011) Laser microdissection of plant-fungus interaction sites and isolation of RNA for downstream expression profiling. Methods in Molecular Biology: Plant Immunity (ed. J. McDowell) 712:241-62, Humana Press, New York. PDF
Chandran, D., Hather, G., and M.C. Wildermuth (2011) Global expression profiling of RNA from laser microdissected cells at fungal–plant interaction sites. Methods in Molecular Biology: Plant Immunity (ed. J. McDowell) 712: 263-81, Humana Press, New York. PDF
Ford, K.A., Casida J.E., Chandran, D., Gulevich, A.G., Okrent, R.A., Durkin, K.A., Sarpong, R., Bunnelle, E.M., and M.C. Wildermuth (2010) Neonicotinoid insecticides induce salicylate-associated plant defense responses. Proc. Natl. Acad. Sci. USA 107: 17527-17532. PDF Supplemental material
M.C. Wildermuth (2010) Modulation of host nuclear ploidy: a common plant biotroph mechanism. Current Opinion in Plant Biology 13: 449-458. PDF
Chandran, D., Inada, N., Hather, G., Kleindt, C.K. and M.C. Wildermuth (2009) Laser microdissection of Arabidopsis cells at the powdery mildew infection site reveals site-specific processes and regulators. Proceedings of the National Academy of Sciences 107: 460-5. Epub Dec 14, 2009. PDF Supplemental material
Okrent, R.A., Brooks, M.D., and M.C. Wildermuth (2009) Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate. Journal of Biological Chemistry 284: 9472-54. PDF
Chandran, D., Tai, Y.C., Hather, G., Dewdney, J., Denoux, C., Burgess, D.G., Ausubel, F.M., Speed, T.P., and M.C. Wildermuth (2009) Temporal global expression data reveals known and novel salicylate-impacted processes and regulators mediating powdery mildew growth and reproduction on Arabidopsis. Plant Physiology 149: 1435-1451. PDF
Zhang, N.R., Wildermuth, M.C., and T.P.Speed (2008) Transcription factor binding site prediction with multivariate gene expression data. Annals of Applied Statistics 2: 332-365. PDF Supplement
Jones, A.M., Lindow, S.E., and M.C. Wildermuth (2007) Salicylic acid, yersiniabactin, and pyoverdin production by the model phytopathogen Pseudomonas syringae pv. tomato DC3000: Synthesis, regulation, and impact on tomato and Arabidopsis host plants. Journal of Bacteriology 189: 6773-6786. PDF Supplement
Nobuta, K., Okrent, R.A., Stoutemyer, M., Rodibaugh, N., Kempema, L., *^Wildermuth, M.C., and R.W. *Innes (2007) The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis. Plant Physiology 144: 1144-1156. * co-senior authors; ^corresponding author. PDF Supplement
Strawn, M.A., Marr, S.K., Inoue, K., Inada, N., Zubieta, C., and M.C. Wildermuth (2006) Arabidopsis isochorismate synthase functional in pathogen-induced salicylate biosynthesis exhibits properties consistent with a role in diverse stress responses. Journal of Biological Chemistry 282: 5919-5933. PDF
M.C. Wildermuth (2006) Variations on a theme: synthesis and modification of plant benzoic acids. Current Opinion in Plant Biology 9: 288-296. PDF
Inada, N. and M.C. Wildermuth (2004) Novel tissue preparation method and cell-specific marker for laser microdissection of Arabidopsis mature leaf. Planta 221:9-16. PDF
Gu, Y.-Q., Wildermuth, M.C., Chakravarthy, S., Loh, Y.-T., Yang, C., He, X., Han, Y., and G.B. Martin (2002) Tomato transcription factors Pti4, Pti5, and Pti6 activate defense responses in Arabidopsis. Plant Cell 14: 817-831. PDF
Wildermuth, M.C., Dewdney, J., Wu, G., and F.M. Ausubel (2001) Isochorismate synthase is required to synthesize salicylic acid for plant defense. Nature 414: 562-565. PDF Supplemental Figure Supplemental Table
Dewdney, J., Reuber, T.L., Wildermuth, M.C., Devoto, A., Cuit, J., Stutius, L.M., Drummond, E.P., and F.M. Ausubel (2000) Three unique mutants of Arabidopsis identify EDS loci required for limiting growth of a biotrophic fungal pathogen. Plant Journal 24: 205-218. PDF
Guenther, A., Archer, S., Greenberg, J., Harley, P., Helmig, D., Klinger, L. Vierling, L., Wildermuth, M., Zimmerman, P., and S. Zitzer (1999) Biogenic hydrocarbon emissions and landcover/climate change in a subtropical savanna. Physics and Chemistry of the Earth 24: 659-667.
Fall, R. and M.C. Wildermuth (1998) Isoprene synthase: from biochemical mechanism to emission algorithm. Journal of Geophysical Research 103: 25,599-25,609.
Wildermuth, M.C. and R. Fall (1998) Biochemical characterization of stromal and thylakoid-bound isoforms of isoprene synthase in willow leaves. Plant Physiology 116: 1111-1121.
Wildermuth, M.C. and R. Fall (1996) Light-dependent isoprene emission: Characterization of a thylakoid-bound isoprene synthase in Salix discolor chloroplasts. Plant Physiology 112: 171-82.
Monson, R.K., Harley, P.C., Litvak, M.E., Wildermuth, M., Guenther, A.B., Zimmerman, P.R., and R. Fall (1994) Environmental and developmental controls over the seasonal pattern of isoprene emission from aspen leaves. Oecologia 99: 260-270.
Guenther, A., Zimmerman, P. and M. Wildermuth (1994) Natural volatile organic compound emission rate estimates for U.S. woodland landscapes. Atmospheric Environment 28: 1197-1210.
Honors and Awards
Winkler Family Foundation Award - Winkler Family Foundation - 2007
Presidential Chair Fellow - University of California, Berkeley - 2005
Neish Young Investigator Award - Phytochemistry Society of North America - 2005
USDA National Research Initiative Award, Postdoctoral Fellowship - USDA - 2000
NIAID Mechanisms in Bacterial Pathogenesis, Postdoctoral Fellowship - Harvard Medical School - 1998