Ph.D. Microbiology Duke University, 1962
B.A. Emory and Henry College, 1958
My current research focuses on thioredoxin and is carried out collaboratively. We study (1) The diversity of thioredoxin and its role in redox regulation, emphasizing anaerobic archaea and bacteria as well as oxygenic photosynthetic prokaryotes, and (2) Applying thioredoxin to improve the quality and yield of cereals.
Thioredoxin (Trx) is a disulfide protein found in all types of living cells. Discovered as an electron donor for ribonucleotide reductase, Trx has since been found to play a regulatory role in a spectrum of processes throughout biology. The protein is also finding a place in medicine and biotechnology.
My Main Projects:
- Diversity of Trx systems with an emphasis on their role in anaerobic archaea and bacteria and oxygenic photosynthetic prokaryotes.
Monica Balsera (CSIC, Salamanca, Spain)
- Improvement in cereal quality and yield by Trx:
Evolution of Redox Regulation
Balmer, Y., Koller, A., del Val, G., Manieri, W., Schürmann, P., Buchanan, B.B. (2003) Proteomics gives insight into the regulatory function of chloroplast thioredoxins. Proc. Natl. Acad. Sci. USA 100, 370-375.
Balsera, M., Uberegui, E., Susanti, D., Schmitz, R.A., Mukhopadhyay, B., Schürmann, P. and Buchanan, B.B. (2012) Ferredoxin:thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria. Planta Epub before print.
Buchanan, B.B., Kalberer, P.P. and Arnon, D.I. (1967) Ferredoxin-activated fructose diphosphatase in isolated chloroplasts. Biochem. Biophys. Res. Commun. 29, 74-79.
Evans, M.C.W., Buchanan, B.B. and Arnon, D.I. (1966) A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium. Proc. Natl. Acad. Sci. USA 55, 928-934.
Holmgren, A. (1979) Thioredoxin catalyzes the reduction of insulin by dithiothreitol and dihydrolipoamide. J. Biol. Chem. 254, 9627-9632.
Hosoya-Matsuda N, Inoue, K and Hisabori T. (2009) Roles of thioredoxin in the obligate anaerobic green sulfur photosynthetic bacterium Chlorobaculum tepidum. Mol. Plant 2, 336-343.
Wolosiuk, R.A. and Buchanan, B.B. (1977) Thioredoxin and glutathione regulate photosynthesis in chloroplasts. Nature 266, 565-567.
Buchanan BB. (2007) Thioredoxin: an unexpected meeting place. Photosynth Res. 92:145-148.
Lee SC, Lan WZ, Kim BG, Li L, Cheong YH, Pandey GK, Lu G, Buchanan BB, Luan S. (2007) A protein phosphorylation/dephosphorylation network regulates a plant potassium channel. Proc Natl Acad Sci U S A. 104:15959-15964.
Fu A, He Z, Cho HS, Lima A, Buchanan BB, Luan S. (2007) A chloroplast cyclophilin functions in the assembly and maintenance of photosystem II in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 104:15947-15952.
Lichtenthaler HK, Buchanan BB, Douce R. (2008) Honoring Andrew Benson in Paris : a tribute on his 90th birthday. Photosynth Res. 96:181-183.
Schürmann P, Buchanan BB. (2008) The ferredoxin/thioredoxin system of oxygenic photosynthesis. Antioxid Redox Signal. 10:1235-1274.
Dominguez-Solis, J. R., He, Z., Lima, A., Ting, J., Buchanan, B.B., Luan S. (2008) A cyclophilin links redox and light signals to cysteine biosynthesis and stress responses in chloroplasts. Proc. Natl. Acad. Sci. USA 105, 16386-16391.
Balsera, M., Goetze, T.A., Kovács-Bogdán, E., Schürmann, P., Wagner, R., Buchanan, B.B., Soll, J., Bölter, B. (2008) Characterization of Tic110, a channel-forming protein at the inner envelope membrane of chloroplasts, unveils a response to Ca2+ and a stromal regulatory disulfide bridge. J. Biol. Chem. 284, 2603-2616.
Wong, J. H., Lau, T., Cai, N., Singh, J., Pedersen, J. F., Vensel, W. H., Hurkman, W. J., Lemaux, P. G. and Buchanan, B. B. (2009) Digestibility of protein and starch from sorghum (Sorghum bicolor) is linked to biochemical and structural features of grain endosperm. J. Cereal Sci. 49, 73-82.
Li, Y., Ren, J., Cho, M.-J., Zhou, S., Kim, Y.-B, Guo, H., Wong, J.H., Niu, H., Kim, H.K., Morigasaki, S. , Lemaux, P.G., Frick, O.L., Yin, J. and Buchanan, B.B. (2009) The level of expression of thioredoxin is linked to fundamental properties and applications of wheat seeds. Mol. Plant 2, 430-441.
Michalska, J., Zauber, H., Buchanan, B.B., Cejudo, F.J. and Geigenberger, P. (2009) NTRC links built-in thioredoxin to light and sucrose in regulating starch synthesis in chloroplasts and amyloplasts. Proc. Natl. Acad. Sci. USA 106, 9908-9913.
Lan W., Lee, S.C., Buchanan, B.B. and Luan, S. (2009) A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells. Proc. Natl. Acad. Sci. USA 106, 21419-21424.
Stengel, A., Benz, J.P., Buchanan, B.B., Soll, J. and Bölter, B. (2009) Preprotein import into chloroplasts via the Toc and Tic complexes is regulated by redox signals. Mol. Plant. 2, 1118-1197.
Meng, L., Wong, J.H., Feldman, L.J., Lemaux, P.G. and Buchanan, B.B. (2010) A membrane-associated thioredoxin required for plant growth moves from cell-to-cell suggestive of a role in intercellular communication. Proc. Natl. Acad. Sci. USA 107, 3900-3905.
Lan, W., Wang, W., Wang, S., Gao, J., Buchanan, B.B., Lin, H., and Luan, S. (2010) A high-affinity potassium transporter (HKT) in rice conceals a calcium-permeable cation channel. Proc. Natl. Acad. Sci. USA. 107, 7089-7094.
Meng, L., Buchanan, B.B., Feldman, L.J. and Luan, S. (2012) CLE-like (CLEL) peptides control the pattern of root growth and lateral root development in Arabidopsis. Proc. Natl. Acad. Sci. USA. 109, 1760-1765.
Meng, L., Buchanan, B.B., Feldman, L.J. and Luan, S. (2012) A Putative Nuclear CLE-Like (CLEL) Peptide Precursor Regulates Root Growth in Arabidopsis. Mol. Plant 5, 955-957.
Wong, J.H., Pedersen, J.F., Buchanan, B.B. and Lemaux, P.G. (2012) Western Blot Analysis Uncovers Clues to Prolamin Digestibility in Raw and Cooked Meal from Sorghum and Corn. Eur. J. Plant Sci. Biotech. 6, 56-65.
Yu, F., Qian, L., Nibau, C., Duan, Q., Kita, D., Levasseur, K., Li, X., Lu, C., Li, H., Hou, C., Li, L., Buchanan, B.B., Chen, L., Cheung, A.Y., Li, D. and Luan, S. (2012) FERONIA Receptor Kinase Pathway Suppresses Abscisic Acid Signaling in Arabidopsis by Activating ABI2 Phosphatase. Proc. Natl. Acad. Sci. USA. 109, 14693-14698.
Balsera, M., Uberegui, E., Susanti, D., Schmitz, R.A., Mukhopadhyay, B., Schürmann, P. and Buchanan, B.B. (2012) Ferredoxin:thioredoxin reductase (FTR) links the regulation of oxygenic photosynthesis to deeply rooted bacteria. Planta Cover Feature of Special Issue
Buchanan, B.B., Wong, J.H. (2012) A conversation with Andrew Benson: Reflections on the discovery of the Calvin-Benson cycle. Photosyn. Res. Epub ahead of print. Link to video on youtube.
Schürmann P. and Buchanan B. B. (2008) The ferredoxin/thioredoxin system of oxygenic photosynthesis. Antioxid. Redox Signal. 10, 1235-1274.
Montrichard, F., Alkhalfioui, F., Yano, H., Vensel, W.H., William J. Hurkman, W. J., and Buchanan, B. B. (2008) Thioredoxin targets in plants: The first 30 years. Proteomics 72, 452-474.
Meyer, Y., Buchanan, B.B, Vignols, F. and Reichheld, J.P. (2009) Thioredoxins and Glutaredoxins: Unifying elements in redox biology. Annu. Rev. Genetics 43, 335-367.
Buchanan, B.B., Holmgren, A., Jacquot, J.-P. and Scheibe, R. (2012) Fifty years in the thioredoxin field and a bountiful harvest. Biochim. Biophys. Acta 1820, 1822-1829.
Special Issues of Journals
Buchanan, B.B., Douce, R. and Lichtenthaler, H K. (2007) Editors, A Tribute to Andrew A. Benson, Special Issue of Photosynthesis Research, Vol. 92, No. 2, pp. 142-271.
Buchanan, B.B, Dietz, K.-J., Geigenberger, P., Jacquot, J.-P., and Schürmann, P. (2009) Editors, Special Issue on Redox Biology. Molecular Plant Vol. 2, No. 2, March 2009
Buchanan, B.B., Dietz, K.-J., Geigenberger, P., Jacquot, J.-P., and Schürmann, P. (2009) Editors, Redox Biology II Special Issue of Molecular Plant. Vol. 2, No. 3, May 2009.
Honors & Awards
Honorary Professor - Henan Agricultural University - Zhengzhou, China - 2007
Fellow - American Society of Plant Biologists - 2007
Fellow - American Academy of Microbiology - 2006
The Stephen Hales Prize - American Society of Plant Biologists - 2005
The William and Martha DeFriece Award - Emory and Henry College - 2005
Who’s Who in America - Marquis - 2004
Book Award - Association Trends - 2001
Silver Book Award - Society of National Association Publications - 2001
Endowed Lecture - Bob B. Buchanan Annual Lecture - Dept. of Plant & Microbial Biology - 2000
Distinguished Achievement Award - Emory & Henry College - 2000
Charles F. Kettering Award for Excellence in Photosynthesis - American Society of Plant Biologists (formerly Physiologists) - 1998
Fellow - American Association for the Advancement of Science - 1997
Fellow - American Academy of Arts and Sciences - 1997
Member - National Academy of Sciences - 1995
Bessenyei Medal - György Bessenyei College & Hungarian Ministry of Education - 1987
Fellowship - Organisation de Cooperation et Developpement Economiques - 1986
Senior Scientist Award - National Science Foundation - 1984
Special Creativity Award - National Science Foundation - 1982
Fellowship - Guggenheim Foundation - 1974
Fellowship - National Institutes of Health Postdoctoral - 1962
Fellowship - National Institutes of Health Predoctoral - 1960
Fellowship - Duke University – 1958-59